If generating v2 sample sheets for instruments besides the ones shown, confirm the ultimate sample sheet has the i5 index in the proper orientation as described higher than.
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Established A Index Adapters Make reference to Index 2 (i5) Orientation For more info regarding how to enter i5 bases about the sample sheet in forward or reverse enhance orientation.
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For Neighborhood Run Supervisor (LRM), input the sequences inside the reverse complement when setting up the operate Therefore the software program quickly makes the ahead orientation as essential for Examination. That is the opposite of most of our advice and is particularly just for the MiniSeq Quick kits.
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This means that these software package work on the idea that the i5 sequence is always from the ahead orientation and can quickly develop the reverse enhance of the sequences presented for the right instrument kind.
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